Influence of Different Cryoprotocols and Strains on the Sperm Viability of FUNAAB Alpha Chickens
Asian Journal of Research in Animal and Veterinary Sciences,
Page 44-54
Abstract
Semen preservation is an essential tool used for a successful application of artificial insemination in livestock industry. Studies on semen quality characteristics on poultry breeds and strains after cryopreservation have been carried out but none has been reported for Nigeria FUNAAB alpha chickens. Three studies were carried out to evaluate the different cryoprotocols on viability of cryopreserved spermatozoa of FUNAAB alpha chickens.
Thirty cocks 25-30 weeks old of normal feather, naked neck and frizzle feather of FUNAAB alpha chickens were used for this experiment. The semen samples were diluted in Tris-based extender. The semen samples were divided into 2 parts. One part washed with normal saline water by centrifuging at 500xg once for 5 minutes in order to remove seminal plasma while the second part was unwashed. Washed and unwashed semen samples were cryopreserved using slow and rapid cryoprotocols, thereafter samples were evaluated for sperm viability. The experiment was laid out in 3x2x2 factorial arrangement. Data obtained were subjected to two-way ANOVA. Higher (p<0.05) motility was observed in washed spermatozoa from Frizzled Feather (FF) subjected to slow freezing (SF) compared to others strains either washed or unwashed, SF or RF protocols. Lower (p<0.05) motility of washed spermatozoa from Normal Feather (NF), Naked Neck (NN) and Frizzled Feather (FF) subjected to rapid freezing (RF) were comparable with washed spermatozoa from NN and unwashed NN subjected to SF and RF. Results for livability, acrosome integrity, seminal Leukocytes and MDA concentration were comparable (p>0.05) for unwashed and washed spermatozoa from NN, NF and FF, subjected to slow and rapid freezing cryoprotocols.
The study concluded that slow and rapid freezing cryoprotocols had a deleterious effect on the spermatozoa of NF, NN, FF and removal of seminal plasma through centrifugation did not improve the viability of the spermatozoa.
Keywords:
- Sperm viability
- FUNAAB alpha chicken
- cryoprotocols
- centrifugation
How to Cite
Ndubuisi-Ogbonna, L. C., Daramola, J. O., Akintunde, A. O., Abdullah, A.-R., Wheto, M., & Afodu, O. J. (2021). Influence of Different Cryoprotocols and Strains on the Sperm Viability of FUNAAB Alpha Chickens. Asian Journal of Research in Animal and Veterinary Sciences, 8(1), 44-54. Retrieved from https://journalajravs.com/index.php/AJRAVS/article/view/30151
References
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Cheng FP, Guo TJ, Wu TJ, Lin TE, Ursem PJF, Colenbrander B, Fung HP. Annual variation in semen characteristics of pigeons (Columba livia). Poultry Science. 2002;81:1050-1056.
Alkan S, Baran A, Ozdas OB, Evecen M. Morphological defects in turkey semen. Turkish Journal of Veterinary & Animal Science. 2002;26:1087-1092.
Tijjani HU, Faisal A, Asmad K. Effect of different glycerol concentrations on sperm quality after preservation of Malaysian indigenous cockerel semen. Savannah Journal of Agriculture. 2014;9(1):56-67.
Long JA. Avian semen cryopreservation: what are the bio-logical challenges? Poult. Sci. 2006;85:232–236.
Peters SO, Omidiji EA, Ikeobi CON, Ozoje MO, Adebambo OAA. Effect of Naked Neck and Frizzled Genes on Egg Traits, Fertility and Hatchability in Local Chicken. 9th Annual Conference of Animal Science Association of Nigeria. 2004;262-264.
Blesbois E, Grasseau I, Seigneurin F. Membrane fluidity and the ability of domestic bird spermatozoa to survive cryopreservation. Reproduction. 2005; 129:371-378.
Hammerstedt RH. Cryopreservation of poultry semen— Current status and economics. In: Proceedings: First International Symposium on the Artificial Insemination of Poultry. M. R. Bakst and G. J. Wishart, ed. Poultry Science Association, Savoy, IL. 1995;229-250.
Etches RJ. Reproduction in Poultry. CAB International, Wallingford, UK. 1996;241-250.
Massip A, Leibo S, Blesbois E. Cryobiology and the breeding of domestic animals B.J. Fuller, N. Lane, E.E. Benson (Eds.), Life in the Frozen State, CRC Press, London, UK. 2004;371-392.
Blesbois E, Labbé C. Main improvements in semen and embryo cryopreservation for fish and fowl. In Cryopreservation of Animal Genetic Resources in Europe, pp 55–66. Ed. D Planchenault. Paris, France: Bureau des Ressources Génétiques; 2003.
Holt WV. Fundamental aspects of sperm cryobiology: the importance of species and individual differences. Theriogenol. 2000;53:47-58.
Blanco JM, Gee G, Wildt DE, Donoghue AM. Species variation in osmotic, cryoprotectant, and cooling rate tolerance in poultry, eagle and Peregrine falcon spermatozoa. Biology of Reproduction. 2000;63:1164-1171.
DOI: 10.1095/biolreprod63.4.1164
Ansah G, Buckland R. Genetic variation in fowl semen cholesterol and phospholipid levels and the relationship of these lipids with fertility of frozen–thawed and fresh semen. Poultry Science. 1982;92:604–615.
Burrows WH, Quinn JP. The collection of spermatozoa from the domestic fowl and turkey. Poultry Science. 1937;24:19-24.
Mphaphathi ML, Luseba D, Sutherland B, Nedambale TL. Comparison of slow freezing and vitrification methods for Venda cockerel’s spermatozoa. Open Journal of Animal Sciences. 2012;2(3): 204-210. Available:http://dx.doi.org/10.4236/ojas.2012.23028
Srirattana K, Sripunya N, Sangmalee A, Imsoonthornruksa S, Liang Y, Ketudat-Cairns M, Parnpai R. Developmental potential of vitrified goat oocytes following somatic cell nuclear transfer and parthenogenetic activation. Small Rumin. Res. 2013;112:141–146.
Tarig AA, Wahid H, Rosnina Y, Yimer N, Goh YM, Baiee FH, Khumran AM, Salman H, Assi MA, Ebrahimi M. Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen. Vet. World. 2017;10(6):672-678.
Available:https://doi.org/10.14202/vetworld.2017.672-678
Bearden HJ, Fuquay. The Male Reproduction System. In: Applied Animals Reproduction 4th Ed. New Jersey: Prentice; 1997.
Ricci G, Perticarari S, Boscolo R, Montico M, Guaschino S, Presani G. Semen preparation methods and sperm apoptosis: swim-up versus gradient-density centrifugation technique. Fertility and Sterility. 2009;91:632-8.
Khan M, Ijaz A. Assessing undiluted, diluted and frozen-thawed Nili-Ravi buffalo bull sperm by using standard semen assays. Italian J Anim Sci. 2007;6:784–787.
Ahmad M, Nasrullah R, Riaz H, Sattar A, Ahmad N. Changes in motility, morphology, plasma membrane and acrosome integrity during stages of cryopreservation of buck sperm. Journal of the South African Veterinary Association. 2014;85:1–5.
Zubair M, Akbar Lodhi L, Ahmad E, Muhammad G. Osmotic swelling test as screening for evaluation of semen of bull. Journal of Entomology and Zoological Studies. 2013;1(6):124-128.
Pintado B, De la Fuente J, Roldan ERS. Permeability of boar and bull spermatozoa to the nucleic acid stains propidium iodide or Hoechet 33258, or to eosin: accuracy in the assessment of cell viability. Journal of Reproduction and Fertility. 2000;118:144-152.
Yagi K. Simple procedure for specific assay of lipid hydroperoxides in serum or plasma. Free Radicals Antioxidant Protocols. 1998;108:101–106.
Makhafola MB, Lehloenya KC, Mphaphathi ML, Dinnyes A, Nedambale TL. The effect of breed on the survivability and motility rate of cryopreserved cock semen. South African Journal of Animal Science. 2009; 39:242-245.
Siudzinska A, Lukaszewicz E. The effect of breed on freezability of semen of fancy fowl. Animal Science Papers and Reports. 2008;26:331-340.
Han XF, Niu ZY, Liu FZ, Yang CS. Effects of diluents, cryoprotectants, equilibration time and thawing temperature on cryopreservation of duck semen. Poultry Science. 2005;4:197-201.
DOI: 10.3923/ijps.2005.197.201
Tabatabaei S, Batavani RA, Talebi AR. Comparism of oviductal sperm age on fertility, hatchability and embryonic death rates in Iranian indigenous and ross-308 broiler breeder chickens. Journal of Animal and Veterinary. 2009;8:85-89.
Omeje SSI, Marine BN. Evaluation of semen characteristics of adult cocks of different genetic background. Theriogenology. 1990;24:1111-1118.
Peters SO, Shoyebo OD, Ilori BM, Ozoje MO, Ikeobi CON, Adebambo AO. Semen quality traits of seven strain of chickens raised in the humid tropics. International Journal of Poultry Science. 2008;7:949-953.
Ajayi FO, Agraviezor BO, Ebogomo D. Comparative studies of semen and haematology quality of Nigeria indigenous and exotic chicken breeds in the humid tropical zone of Nigeria. Global Journal of Bio – Science and Biotechnology. 2014;3(2):164–168.
Ibe SN. Improving Productive Adaptability of the Nigerian local chicken. Proceedings Silver. Anniv. Conference, NSAP/WASAP, Abeokuta: University of Agriculture. 1988; 460–465.
Santiago-Moreno J, Bernal B, Pérez-Cerezales S, Castaño C, Toledano Diaz A, Esteso MC, Gutiérrez-Adán A, López-Sebastián A, Gil MG, Woelders H, Blesbois E. Seminal plasma amino acid profile in different breeds of chicken: Role of seminal plasma on sperm cryoresistance. PLoS One. 2019;14: 1-19.
Blesbois E, de Reviers M. Effect of different fractions of seminal plasma on the fertilizing ability of fowl spermatozoa stored in vitro. J. Reprod. Fertil. 1992;95: 263–8.
Douard V, Hermier D, Labbe C, Magistrini M, Blesbois E. Role of seminal plasma in damage to turkey spermatozoa during in vitro storage. Theriogenology. 2005;63: 126–137.
Long JA. 028 Successful cryopreservation of avian germplasm: Why a multifaceted approach is required. Cryobiology. 2013;67(3):406. Available:https://doi.org/10.1016/j.cryobiol.2013.09.034.
Ejaz R, Ansari MS, Rakha BA, Ullah N, Husna AU, Iqbal R, Akhter S. Arachidic acid in extender improves post-thaw parameters of cryopreserved Nili-Ravi buffalo bull semen. Reprod Domest Anim. 2014;49(1):122–125.
DOI: 10.1111/rda.12239.
Giraud MN, Motta C, Boucher D, Grizard G. Membrane fluidity predicts the outcome of cryopreservation of human spermatozoa. Human Reproduction. 2000; 15(10):2160-2164.
Gomez FP, Park R, Viana AG, Fernandez Costa C, Topper E, Kaya A, Memili E, Yates JR, Moura AA. Protein signatures of seminal plasma from bulls with contrasting frozen-thawed sperm viability. Sci. Rep. 2020;10:14661.
Available:https://doi.org/10.1038/s41598-020-71015-9
Rosato M, Iaffaldano N. Cryopreservation of rabbit semen: Comparing the effects of different cryoprotectants, cryoprotectantfree vitrification, and the use of albumin plus osmoprotectants on sperm survival and fertility after standard vapor freezing and vitrification. Theriogenology. 2013;79:508–516.
Cheng FP, Guo TJ, Wu TJ, Lin TE, Ursem PJF, Colenbrander B, Fung HP. Annual variation in semen characteristics of pigeons (Columba livia). Poultry Science. 2002;81:1050-1056.
Alkan S, Baran A, Ozdas OB, Evecen M. Morphological defects in turkey semen. Turkish Journal of Veterinary & Animal Science. 2002;26:1087-1092.
Tijjani HU, Faisal A, Asmad K. Effect of different glycerol concentrations on sperm quality after preservation of Malaysian indigenous cockerel semen. Savannah Journal of Agriculture. 2014;9(1):56-67.
Long JA. Avian semen cryopreservation: what are the bio-logical challenges? Poult. Sci. 2006;85:232–236.
Peters SO, Omidiji EA, Ikeobi CON, Ozoje MO, Adebambo OAA. Effect of Naked Neck and Frizzled Genes on Egg Traits, Fertility and Hatchability in Local Chicken. 9th Annual Conference of Animal Science Association of Nigeria. 2004;262-264.
Blesbois E, Grasseau I, Seigneurin F. Membrane fluidity and the ability of domestic bird spermatozoa to survive cryopreservation. Reproduction. 2005; 129:371-378.
Hammerstedt RH. Cryopreservation of poultry semen— Current status and economics. In: Proceedings: First International Symposium on the Artificial Insemination of Poultry. M. R. Bakst and G. J. Wishart, ed. Poultry Science Association, Savoy, IL. 1995;229-250.
Etches RJ. Reproduction in Poultry. CAB International, Wallingford, UK. 1996;241-250.
Massip A, Leibo S, Blesbois E. Cryobiology and the breeding of domestic animals B.J. Fuller, N. Lane, E.E. Benson (Eds.), Life in the Frozen State, CRC Press, London, UK. 2004;371-392.
Blesbois E, Labbé C. Main improvements in semen and embryo cryopreservation for fish and fowl. In Cryopreservation of Animal Genetic Resources in Europe, pp 55–66. Ed. D Planchenault. Paris, France: Bureau des Ressources Génétiques; 2003.
Holt WV. Fundamental aspects of sperm cryobiology: the importance of species and individual differences. Theriogenol. 2000;53:47-58.
Blanco JM, Gee G, Wildt DE, Donoghue AM. Species variation in osmotic, cryoprotectant, and cooling rate tolerance in poultry, eagle and Peregrine falcon spermatozoa. Biology of Reproduction. 2000;63:1164-1171.
DOI: 10.1095/biolreprod63.4.1164
Ansah G, Buckland R. Genetic variation in fowl semen cholesterol and phospholipid levels and the relationship of these lipids with fertility of frozen–thawed and fresh semen. Poultry Science. 1982;92:604–615.
Burrows WH, Quinn JP. The collection of spermatozoa from the domestic fowl and turkey. Poultry Science. 1937;24:19-24.
Mphaphathi ML, Luseba D, Sutherland B, Nedambale TL. Comparison of slow freezing and vitrification methods for Venda cockerel’s spermatozoa. Open Journal of Animal Sciences. 2012;2(3): 204-210. Available:http://dx.doi.org/10.4236/ojas.2012.23028
Srirattana K, Sripunya N, Sangmalee A, Imsoonthornruksa S, Liang Y, Ketudat-Cairns M, Parnpai R. Developmental potential of vitrified goat oocytes following somatic cell nuclear transfer and parthenogenetic activation. Small Rumin. Res. 2013;112:141–146.
Tarig AA, Wahid H, Rosnina Y, Yimer N, Goh YM, Baiee FH, Khumran AM, Salman H, Assi MA, Ebrahimi M. Effect of different concentrations of soybean lecithin and virgin coconut oil in Tris-based extender on the quality of chilled and frozen-thawed bull semen. Vet. World. 2017;10(6):672-678.
Available:https://doi.org/10.14202/vetworld.2017.672-678
Bearden HJ, Fuquay. The Male Reproduction System. In: Applied Animals Reproduction 4th Ed. New Jersey: Prentice; 1997.
Ricci G, Perticarari S, Boscolo R, Montico M, Guaschino S, Presani G. Semen preparation methods and sperm apoptosis: swim-up versus gradient-density centrifugation technique. Fertility and Sterility. 2009;91:632-8.
Khan M, Ijaz A. Assessing undiluted, diluted and frozen-thawed Nili-Ravi buffalo bull sperm by using standard semen assays. Italian J Anim Sci. 2007;6:784–787.
Ahmad M, Nasrullah R, Riaz H, Sattar A, Ahmad N. Changes in motility, morphology, plasma membrane and acrosome integrity during stages of cryopreservation of buck sperm. Journal of the South African Veterinary Association. 2014;85:1–5.
Zubair M, Akbar Lodhi L, Ahmad E, Muhammad G. Osmotic swelling test as screening for evaluation of semen of bull. Journal of Entomology and Zoological Studies. 2013;1(6):124-128.
Pintado B, De la Fuente J, Roldan ERS. Permeability of boar and bull spermatozoa to the nucleic acid stains propidium iodide or Hoechet 33258, or to eosin: accuracy in the assessment of cell viability. Journal of Reproduction and Fertility. 2000;118:144-152.
Yagi K. Simple procedure for specific assay of lipid hydroperoxides in serum or plasma. Free Radicals Antioxidant Protocols. 1998;108:101–106.
Makhafola MB, Lehloenya KC, Mphaphathi ML, Dinnyes A, Nedambale TL. The effect of breed on the survivability and motility rate of cryopreserved cock semen. South African Journal of Animal Science. 2009; 39:242-245.
Siudzinska A, Lukaszewicz E. The effect of breed on freezability of semen of fancy fowl. Animal Science Papers and Reports. 2008;26:331-340.
Han XF, Niu ZY, Liu FZ, Yang CS. Effects of diluents, cryoprotectants, equilibration time and thawing temperature on cryopreservation of duck semen. Poultry Science. 2005;4:197-201.
DOI: 10.3923/ijps.2005.197.201
Tabatabaei S, Batavani RA, Talebi AR. Comparism of oviductal sperm age on fertility, hatchability and embryonic death rates in Iranian indigenous and ross-308 broiler breeder chickens. Journal of Animal and Veterinary. 2009;8:85-89.
Omeje SSI, Marine BN. Evaluation of semen characteristics of adult cocks of different genetic background. Theriogenology. 1990;24:1111-1118.
Peters SO, Shoyebo OD, Ilori BM, Ozoje MO, Ikeobi CON, Adebambo AO. Semen quality traits of seven strain of chickens raised in the humid tropics. International Journal of Poultry Science. 2008;7:949-953.
Ajayi FO, Agraviezor BO, Ebogomo D. Comparative studies of semen and haematology quality of Nigeria indigenous and exotic chicken breeds in the humid tropical zone of Nigeria. Global Journal of Bio – Science and Biotechnology. 2014;3(2):164–168.
Ibe SN. Improving Productive Adaptability of the Nigerian local chicken. Proceedings Silver. Anniv. Conference, NSAP/WASAP, Abeokuta: University of Agriculture. 1988; 460–465.
Santiago-Moreno J, Bernal B, Pérez-Cerezales S, Castaño C, Toledano Diaz A, Esteso MC, Gutiérrez-Adán A, López-Sebastián A, Gil MG, Woelders H, Blesbois E. Seminal plasma amino acid profile in different breeds of chicken: Role of seminal plasma on sperm cryoresistance. PLoS One. 2019;14: 1-19.
Blesbois E, de Reviers M. Effect of different fractions of seminal plasma on the fertilizing ability of fowl spermatozoa stored in vitro. J. Reprod. Fertil. 1992;95: 263–8.
Douard V, Hermier D, Labbe C, Magistrini M, Blesbois E. Role of seminal plasma in damage to turkey spermatozoa during in vitro storage. Theriogenology. 2005;63: 126–137.
Long JA. 028 Successful cryopreservation of avian germplasm: Why a multifaceted approach is required. Cryobiology. 2013;67(3):406. Available:https://doi.org/10.1016/j.cryobiol.2013.09.034.
Ejaz R, Ansari MS, Rakha BA, Ullah N, Husna AU, Iqbal R, Akhter S. Arachidic acid in extender improves post-thaw parameters of cryopreserved Nili-Ravi buffalo bull semen. Reprod Domest Anim. 2014;49(1):122–125.
DOI: 10.1111/rda.12239.
Giraud MN, Motta C, Boucher D, Grizard G. Membrane fluidity predicts the outcome of cryopreservation of human spermatozoa. Human Reproduction. 2000; 15(10):2160-2164.
Gomez FP, Park R, Viana AG, Fernandez Costa C, Topper E, Kaya A, Memili E, Yates JR, Moura AA. Protein signatures of seminal plasma from bulls with contrasting frozen-thawed sperm viability. Sci. Rep. 2020;10:14661.
Available:https://doi.org/10.1038/s41598-020-71015-9
Rosato M, Iaffaldano N. Cryopreservation of rabbit semen: Comparing the effects of different cryoprotectants, cryoprotectantfree vitrification, and the use of albumin plus osmoprotectants on sperm survival and fertility after standard vapor freezing and vitrification. Theriogenology. 2013;79:508–516.
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