Determination of Carbapenem and Extended Spectrum Beta Lactamases in E. Coli from Commercial Broilers, Sri Lanka
Asian Journal of Research in Animal and Veterinary Sciences,
Association between antimicrobial resistance in human and livestock have been widely discussed. The same or closely associated sequence type of E. coli has been reported in poultry and causing clinical infection in humans. The objective of this study was to determine phenotypic and genotypic antimicrobial resistance among E coli isolated from commercial broiler integrators (n=6) in the country. Ceacal samples (n=521) were collected from commercial broiler processing plants and E.coli was isolated and identified by conventional bacteriological methods followed by selected biochemical tests. Antimicrobial susceptibility testing and interpretation were done by disk diffusion tests as described in EUCAST. The conventional PCR tests were carried out for CTX-M for ESBL phenotypically resistant isolates and NDM for meropenem resistance isolates. NDM and CTX-M were found in 0.38% and 18.2 % in E. coli respectively. High frequency of phenotypic resistance was observed against neomycin (100%), tetracycline (99%), ampicillin/amoxycillin (98%), and quinolone (91.5%), gentamicin (79%), and over 50% of the frequency of antimicrobial resistance were shown against amoxycillin + Clavulanic acid, streptomycin and chloramphenicol. All these classes of antimicrobial are widely used in commercial broiler operations in the country. NDM has not been reported in E. coli from commercial poultry previously, although NDM had been reported in human clinical isolates. Prudent usage of antimicrobials, strengthening resistance surveillance, molecular epidemiological studies, and understanding the role of the mobile genetic elements are strongly recommended to minimize the risk of dissemination of antimicrobial resistance in humans.
- E. coli
- Sri Lanka
How to Cite
Vinueza-Burgos C, O.-P.D., Narváez C, De Zutter L, Zurita J. Characterization of cefotaxime resistant Escherichia coli isolated from broiler farms in Ecuador. PLoS One. 2019;14:e0207567.
Gajdács M. The Concept of an Ideal Antibiotic: Implications for Drug Design. Molecules. 2019;24:892.
Suay-García BP.-G, MT. Present and Future of Carbapenem-resistant Enterobacteriaceae (CRE) Infections. Antibiotics. 2019;8:122.
Tillekeratne LG, Vidanagama D, Tippalagama R, Lewkebandara R, Joyce M, Nicholson BP. et al. Extended-spectrum ß-Lactamase-producing Enterobacteriaceae as a Common Cause of Urinary Tract Infections in Sri Lanka. Infect Chemother. 2016;48:160-165.
Tissera K, Liyanapathirana V, Dissanayake N, Pinto V, Ekanayake A, Tennakoon M, et al. Spread of resistant gram negatives in a Sri Lankan intensive care unit. BMC Infectious Diseases. 2004;17:490.
Dortet L, Poirel L, Nordmann P. Worldwide dissemination of the NDM-type carbapenemases in Gram-negative bacteria. BioMed research international. 2014;249856.
Gajdács M, Ábrók M, Lázár A, Jánvári L, Tóth Á, Terhes G, et al. Detection of VIM, NDM and OXA-48 producing carbapenem resistant Enterobacterales among clinical isolates in Southern Hungary. Acta microbiologica et immunologica Hungarica; 2020b.
Yassin AK, Gong J, Kelly P, Lu G, Guardabassi L, Wei L, et al. Antimicrobial resistance in clinical Escherichia coli isolates from poultry and livestock, China. PLoS One. 2004;12: e0185326.
Sleman SM, BA, SwickPaul A. Iji, Specialized protein products in broiler chicken nutrition: A review. Animal Nutrition. 2015;1:47-53.
Zhang R, Li J, Wang Y, Shen J, Shen Z, Wang S. Presence of NDM in non-E. coli Enterobacteriaceae in the poultry production environment. Journal of Antimicrobial Chemotherapy. 2019;74:2209-2213.
Maciuca IE, Williams NJ, Tuchilus C, Dorneanu O, Guguianu E, Carp-Carare C, et al. High Prevalence of Escherichia coli-Producing CTX-M-15 Extended-Spectrum Beta-Lactamases in Poultry and Human Clinical Isolates in Romania. Microb Drug Resist. 2015;21:651-662.
PJ. Quinn PJQ, Carter ME, Bryan Markey GR. Carter, Clinical Veterinary Microbiology. Wolfe; 1994.
Gajdács M, Ábrók M, Lázár A, Burián K. Differential epidemiology and antibiotic resistance of lactose-fermenting and non-fermenting Escherichia coli: Is it just a matter of taste? Biologia Futura. 2020a;71:175-182.
Pitout JDD, Hossain A, Hanson ND. Phenotypic and molecular detection of CTX-M-β-lactamases produced by Escherichia coli and Klebsiella spp. Journal of Clinical Microbiology. 2004;42:5715-5721.
Chelsie N.Geyer, NDH, Rapid PCR amplification protocols decrease the turn-around time for detection of antibiotic resistance genes in Gram-negative pathogens. Disease. 2013;77:13-117.
Roth N, KA, Mayrhofer S, Zitz U, Hofacre C, Domig KJ. The application of antibiotics in broiler production and the resulting antibiotic resistance in Escherichia coli: A global overview. Poultry Sci. 2019;98:1791-1804.
Kuenzli E, Jaeger VK, Frei R, Neumayr A, DeCrom S, Haller S, et al. High colonization rates of extended-spectrum β-lactamase (ESBL)-producing Escherichia coliin Swiss Travellers to South Asia– a prospective observational multicentre cohort study looking at epidemiology, microbiology and risk factors. BMC Infectious Diseases. 2014;14:528.
Nicolas-Chanoine MH BX, Madec JY. Escherichia coli ST131, an intriguing clonal group. Clin Microbiol Rev. 2014;27:543-574.
Gajdács M, Albericio F. Antibiotic Resistance: From the Bench to Patients. Antibiotics (Basel). 2019; 8:129.
Liyanage,GY.a.PM. Risk of prophylactic antibiotics in livestock and poultry farms; a growing problem for human and animal health. Pharmaceutical Journal of Sri Lanka. 2004;7:13-22.
Nhung NT CN, Carrique-Mas JJ. Antimicrobial Resistance in Bacterial Poultry Pathogens: A Review. Front Vet Sci. 2004;4.
Saliu EM VW, Zentek J. Types and prevalence of extended-spectrum beta-lactamase producing Enterobacteriaceae in poultry. Anim Health Res Rev. 2004;18:46-57.
Vounba P, Arsenault J, Bada-Alambédji R, Fairbrother JM, Antimicrobial Resistance and Potential Pathogenicity of Escherichia coli Isolates from Healthy Broilers in Québec, Canada. Microb Drug Resist. 2019;25:1111-1121.
Tang B CJ, Cao L. Characterization of an NDM-5 carbapenemase-producing Escherichia coli ST156 isolate from a poultry farm in Zhejiang, China. BMC Microbiol. 2019;19:82.
Paskova V, Medvecky Matej, Skalova Anna, Chudejova Katerina, Bitar Ibrahim, Jakubu Vladislav, et al. Characterization of NDM-Encoding Plasmids From Enterobacteriaceae Recovered From Czech Hospitals. In Frontiers in microbiology. 2018;1549.
Olsen RH BM, Löhren U, Robineau B, Christensen H. Extended-spectrum β-lactamase-producing Escherichia coli isolated from poultry: a review of current problems, illustrated with some laboratory findings. Avian Pathol. 2014;43:199-208.
ARSP Working Group, T.S.L.C.o.M. A multi centre laboratory study of Gram negative bacterial blood stream infections in Sri Lanka. Ceylon Medical Journal. 2013;58:56-61.
Fernando MMPSC, Luke WANV, Miththinda JKND, Wickramasinghe RDSS, Sebastiampillai BS, Gunathilake MPML, et al. Extended spectrum beta lactamase producing organisms causing urinary tract infections in Sri Lanka and their antibiotic susceptibility pattern –A hospital based cross sectional study. BMC Infectious Diseases. 2004;17:138.
Hawkey PM, Warren RE, Livermore DM, McNulty CAM, Enoch DA, Otter JA, et al. Treatment of infections caused by multidrug-resistant Gram-negative bacteria: report of the British Society for Antimicrobial Chemotherapy/Healthcare Infection Society/British Infection Association Joint Working Party†. Journal of Antimicrobial Chemotherapy. 2018;73:iii2-iii78.
Ruidong Zhaia, BFXS, Chengtao Suna Zhihai Liuc , Shaolin Wanga, Zhangqi Shena, Timothy R. Walsh, Chang Caide, Yang Wang, Congming Wu. Contaminated in-house environment contributes to the persistence and transmission of NDM-producing bacteria in a Chinese poultry farmAuthor links open overlay panel Enviromental International. 2020;139.
Kaushik P, Anjay A, Kumari S, Dayal S, Kumar S. Antimicrobial resistance and molecular characterisation of E. coli from poultry in Eastern India. Veterinaria italiana. 2018;54:197-204.
Herath EM, Palansooriya AGKN, Dandeniya WS, Jinadasa R. An assessment of antibiotic resistant bacteria in poultry litter and agricultural soils in Kandy district, Sri Lanka. Tropical Agricultural Research. 2016;27:389-398.
Alvarez-Fernández E, Domínguez-Rodríguez J, Capita R, Alonso-Calleja C. Influence of housing systems on microbial load and antimicrobial resistance patterns of Escherichia coli isolates from eggs produced for human consumption. J Food Prot. 2012;75:847-853.
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