Ascorbic Acid Effect on Glyphosate-induced Haematological and Serological Pathology in Juveniles of the Catfish Clarias gariepinus (Pisces: Clariidae) Burchell, 1822
Asian Journal of Research in Animal and Veterinary Sciences,
Aims: The study was to determine the effects of sub-lethal concentrations of glyphosate-based herbicide (Delsate®) on blood parameters, serum enzymes and urea of Clarias gariepinus juveniles as well as therapeutic effect of Vitamin C(Kepro®)on the glyphosate-induced pathology.
Study Design: Latin square.
Place and Duration of Study: Department of Fisheries and Aquaculture Management, Nnamdi Azikiwe University Awka. Nigeria, between December 2018 and April 2019.
Methodology: A 48 hours-acute toxicity tests were initially done to determine the respective LC50 of Delsate® and Kepro® using 8 C. gariepinus juveniles of mean weight 41.50±1.35 g and mean length 20.75±0.43 cm. Thereafter one group of C. gariepinus juveniles (n=144) was exposed to 0, 5, 10 and 15mgL⁻¹ sub-lethal concentrations of Delsate® for 91 days followed by different treatments with 50mgL⁻¹ and 100mg L⁻¹ of the vitamin C after 7 days post exposure to glyphosate. Another group of C. gariepinus juveniles (n=144) were exposed concurrently to glyphosate and vitamin C for 91 days.
Results: The LC50 of Delsate® was 75 mgL⁻¹ and Kepro® 175 mgL⁻¹. There was significant decrease (P<.05) in PCV, Hb, RBC and AST of glyphosate-exposed groups when compared with Control. No significant difference occurred between TWBC, DWBC and ALP of exposed and control groups, except in neutrophils where significant increase occurred in ALT and urea. Treatment with 50 and 100mgL⁻¹ vitamin C in glyphosate-exposed groups showed significant increase in PCV, Hb, RBC and ALP with a decrease in mean AST, ALT and Urea The 100 mgL⁻¹ produced better therapeutic benefit than 50 mgL⁻¹ vitamin C. However, concurrent exposure to glyphosate and vitamin C indicated no significant therapeutic effect on the tested blood and serum parameters.
Conclusion: The LC50 of Delsate® and Kepro® for catfish have been determined. Delsate® toxicity induced perturbations in some haematological and biochemical parameters in fish. The level of ascorbic acid (100 mgL⁻¹ Kepro®) used in this study enhances catfish tolerance to environmental stress and could reduce Delsate® toxicity.
- haematological and biochemical perturbations
- vitamin C-treatment.
How to Cite
WHO. Glyphosate. Environmental Health Criteria Publication No. 159, the World Health Organization.Geneva Switzerland; 1994.
Pérez GL, Torremorell A, Mugni H, Rodríguez P, Vera MS, De Nascimento M, Allende L, Bustingorry J, Escaray R, Ferraro M, Izaguirre I, Pizarro H, Bonetto C, Morris DP, Zagarese H. Effects of the herbicide Roundup on freshwater microbial communities: a mesocosm study. Ecol. Appl. 2011;17:2310–2322.
Woodburn AT. Glyphosate: production, pricing and use worldwide. Pest Manage. Sci. 2000;56:309-312.
Okomoda VT, Ataguba GA, Ayuba VO. Hematological response of Clarias gariepinus fingerlings exposed to acute concentrations of Sunsate®. Journal of Stress Physiology and Biochemistry. 2013;9(2):271-278.
Haux C, Larson A, Lithner G, Sjobeck M. A field study of physiological effects on fish in lead- contaminated lakes. Environmental Toxicology and Chemistry. 1986;5:283-288.
Hemming JM, Waller WT, Chow MC, Denslow ND, Venables B. Assessment of the estrogenicity and toxicity of a domestic waste water effluent flowing through a constructed wetland system using biomarkers in male fathead minnows (Pimephalespromelas). Environmental Toxicology and Chemistry. 2001;20:2268-2275.
Maheswaran R, Devapaul A, Velmurugan B, Ignacimuthu S. Haematological studies of freshwater fish Clarias batrachus exposed to mercuric chloride. International Journal of Integrated Biology. 2008;2(1):49-54.
Udem SC, Asogwa O. Effects on haematological and biochemical parameters in albino mice fed Ipomoea batatas leaf aqueous extract. Comprehensive Clinical Pathology. 2011;20:475-479.
Tavares-Dias M, Barcellos JFM. Peripheral blood cells of the armored catfish Hoplosternumlittorate (Hancock, 1828); a morphological and cytochemical study. Brazillian Journal of Morphological Science. 2005;22:215-220.
Winsto GW, Di Giulio RT. Prooxidant and antioxidant mechanisms in aquatic organisms. Aquat Toxicol.1991;19:137–161.
Chatterjee IB, Majunder AK, Nandi BK, Subramadian N. Synthesis and some major functions of vitamin C in animals” Ann .NY Acad. Sci. 1995;258:24-47.
Enegide C, Arome C, Solomon A. A new method for determining acute toxicity in animal models. Toxicology International. 2013;20(3):224-226.
Thrall MA, Weiser MG. Hematology in Hendrix. C.M editor Laboratory procedures for Veterinary Technicians. 4th edition Missouri: Mosby Inc: 2002;29-74.
Higgins T, Beutler E, Doumas B. Measurement of haemoglobin in blood in Tietz fundamentals of Clinical chemistry, 6th edition CA. Burtis, ER Ashwood and DE Bruns. Saint Louis: M.O Sanders Elsevier. S24-52S. 2008;110-115.
Coles EH. Veterinary Clinical Pathology (4th edition).Saunders, W.B. Company Philadephia; 1986.
Bush BM. Interpretation of laboratory results for small animal clinician Blackwell Scientific London; 1991.
Okomoda J, Ayuba VO, Omeji S. Hematological changes of Clarias gariepinus fingerlings exposed to acute toxicity of Formalin. Publication of Nasarawa State University, Keffi. PAT Patnsuk Journal.net; 2010.
Aderolu AZ, Ayoola SO, Otitoloju AA. Effects of acute and sublethal concentrations of Actellic on weight changes and haematology parameters of Clarias gariepinus.World Journal of Biological Research. 2010;3:30-39.
Glusczak L, Miron DS, Crestani M, Dafonseca MB, Pedron FDA, Duarte MF, Vieira VUP. Effects of glyphosate herbicide on acetylcholinesterase activity and metabolic and haematological parameters in Piava (Leporinusobtusiders).Ecotoxicol. Environ. Safety. 2006;65:237-241.
Adeyemo OK. Haematological and Histopathological effects of cassava mill effluent in Clarias gariepinus. African Journal of Biomedical Research. 2005;8:179-183.
Kori-Siakpere O, Ogbe MG, Ikomi RB. Haematological response of the African catfish Clarias gariepinus to sublethal concentrations of Potassium permanganate, Scientific Research and Essay. 2009;4(5):457-466.
Ayuba VO, Ofojekwu PC, Francis A. Growth and haematological changes in C. gariepinus fingerlings exposed to Daturaimoxia seed extract for 12 weeks on weight gain in the African catfish,Annals of Research in Nigeria. 2008;6:46-55.
Joshi P, Deep H. Effect of Lindane and malathion exposure to certain blood parameters in a freshwater teleost fish Clarias batrachus. Poel. Res. 2002;21:55-57.
Neelima P, Sunitha K, Gopala RN, Rao JCS. Haematologicalalterations in Cyprinus carpio as biomarkers of cypermethrin toxicity. Int. J. Curr. Res. 2015;7(8):18864-18870.
Khartun MA, Rahman MR, Islam MS. Effects of cypermethrin and diazinon on haematology of Labeorohita. Int. J. Develp. Res. 2014;4(5):953-957.
Akinrotimi OA, Gabriel UU, Ariweriokuma SV. Haematoxicity of cypermethrin to African catfish Clarias gariepinus under laboratory conditions. Journal of Environmental Engineering and Technology. 2012;1(2):20-25.
Jasper R, Locatelli GO, Pilati C, Locatelli C. Evaluation of biochemical, haematological and oxidative parameters in mice exposed to the herbicide, glyphosate- Round up®. Interdisciplinary Toxicology. 2012; 5(3):133-140. DOI:10.2478/V10102-012-0022-5.
Alfredo S, Roberto S. Gendusa C. In vitro evaluation of genomic damage induced by glyphosate on human lymphocytes.Environmental Science and Pollution Research. 2018;25:34693-34700.
Kondera E, Teodorczuk B, Lugowska K, Witeska M. Effect of glyphosate –based herbicide on haematological and hemopoietic parameters in common carp Cyprinus carpio. Fish Physiology and Biochemistry. 2018;44(3):1011-1018.
Prasad G, Charles S. Haematology and leucocyte enzyme cytochemistry of a threatened yellow catfish Horabagrusbrachysoma. Fish Physiology and Biochemistry. 2010;36(3):435-443.
Cazenave J, Wunderlin DA, Hued AC,Bistoni MDA. Haematological parameters in a neotropical fish, Corydoraspaleatus (Jenyns, 1842) (Pisces, Callichthyidae) captured from pristine and polluted water. Hydrobiologia. 2005;537:25–33.
Clair A, Mesnage R, Travert C, Seralini GEA. Glyphosate based herbicide induces necrosis and apoptosis in mature rat testicular cells in vitro and testosterone decrease at lower levels. Toxicology in vitro. 2012;26(2):269-279.
Jiraungkoorskul W, Uptham ES, Kruatrachue M, Sahaphong SVichasrigrams, Okethitiyook.Biochemical and Histopathological effects of glyphosate herbicide on Nile tilapia Oreochromis niloticus.Environ.Toxicol. 2003;8(4):260-267.
Jyothi B. Narayan G. Certain pesticide–induced carbohydrate metabolic disorders in the serum of freshwater fish Clarias batrachus. Food Chem. Toxicol. 1999;37:417-42.
Udeh GN, Oti EE. Behavioural and some physico-chemical assessments of the freshwater catfish, Clarias gariepinus exposed to acute concentrations of Delsate® herbicide (Glyphosate). Journal of Aquatic Sciences. 2014;29(2A):275-283.
AttiaHala. Hepatoprotective effect of Rubusidaeus leaves against CCL4 – induced liver injury via antioxidant, anti-inflammatory and antipoptic mechanism. European Journal of Medicinal Plants. 2016;14(1):1-16.
Sanchez-Moreno C, Larraun JA. Main methods used in lipid oxidation determination. Food Science and Technology International. 1998;4:391- 399.
Adel ME Shalaby, Abo-Hammad Abbassa. The opposing effect of ascorbic acid (vitamin C) on ochratoxin toxicity in Nile tilapia.(Oreochromis miloticus). Acta Polenica. 2009;2:18-22.
Korkmaz N, Cengiz EI, Unlu E, Uysal E, Yanar M. Cypermetrin-induced Histopathological and biochemical changes in Nile Tilapia (Oreochromis niloticus),and the protective and recuperative effect of ascorbic acid. Environmental Toxicology and Pharmacology. 2009;28:198-205.
Youness ER, Agha FE, El-Toukhy ES, El-Naggars MM, Selim AAI, Ibrahim, AMM. The protective effect of orange juice on glyphosate toxicity in adult male mice. Journal of Chemical and Pharmaceutical Research. 2016;8(2):13-28.
Abstract View: 214 times
PDF Download: 169 times