Enumeration of E. coli, Coliform and Aerobic Bacteria in Marine Fish in Port Sudan, Red Sea State, Sudan

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Iman Homeeda Abd Alrahman Ali
Adil M. A. Salman
El Ayis A. Abubaker
Eman M. Hamad


This study was carried out to detect the total bacterial count, coliform count and E. coli count through the production chain of marine fish (fish market, scaling, restaurant before cooking, restaurant after cooking  and cooking and cooling for 24 h)  in Port Sudan, Red Sea State, during the period from September to December 2018. A total of 50 fish samples were randomly selected from marine fish chain. Samples were subjected for bacteriological examination. The study showed that the mean count of E. coli in the five stages of the fish chain was 2.57×102 +0 .6 cfu, the mean coliform count was 2.01 ×103+ 0 .7 cfu/ g and the mean Aerobic Plate Count (APC) was found to be 3.6×106+ 0.09 cfu/ g. E. coli showed higher mean count (7.58×102) cfu/ g in the scaling stage. The highest aerobic plate count (117×106) cfu/ g was detected also in the scaling  stage, this may be due to the high number of workers involved in this process without using proper hygienic measurements. The highest coliform count 53.9×102 cfu/ g was detected in the stage of precooking, during this period fish were put on the surface of tables without being cooled. Since high coliform contamination takes place after evisceration, the count was in its highest limit after evisceration in the restaurants. By using ANOVA with confidence interval 95% there was a significant difference between the mean of coliform, APC and E. coli count among the five stages in fish production chain. In conclusion the results showed that the marine fish in Port Sudan city were grossly contaminated by E. coli and other coliform, but adequate cooking can kill all the E. coli and coliform.

E. coli, fish, coliform, bacterial count.

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Ali, I. H. A. A., Salman, A. M. A., Abubaker, E. A. A., & Hamad, E. M. (2020). Enumeration of E. coli, Coliform and Aerobic Bacteria in Marine Fish in Port Sudan, Red Sea State, Sudan. Asian Journal of Research in Animal and Veterinary Sciences, 6(3), 9-14. Retrieved from https://journalajravs.com/index.php/AJRAVS/article/view/30113
Original Research Article


Emikpe BO, Adebisi T, Adedeji OB. Bacteria load on the skin and stomach of Clarias gariepinus and Oreochromis niloticus from Ibadan, South West Nigeria: Public health implications. J. Microbiol. Biotech. Res. 2011;1:52-59.

Mahendra Pal, Asnake Ketema, Manyazewal Anberber, Selamawit Mulu, Yashodhara Dutta. Microbial quality of fish and fish products. Beverage and Food World. 2016;43(2).

Arafat. Microbiological assessment of three types of fresh fish (Tilapia niloticus, Labeo niloticus and Hydrocynus spp.) Sold in Ed Dueim, Sudan. New York. Sci. J. 2013; 6(4).

Adebayo-Tayo AC, Odu NN, Michael MU, Okonko IO. Multi-drug resistant (mdr) organisms isolated from sea-foods in Uyo, South-Southern Nigeria. Nature and Sci. 2012;10:61-70.

Ogden ID, Watt AJ. An evaluation of fluorogenic and chromogenic assay for direct enumeration of E. coli. Letters in applied. Microbiol. 1991;13:212-215.

FAO. Practical framework of value chain approach to animal diseases; 2011.

Evelyn Ivana Trindade Damasceno, Lawana Natasha da Gama pantoja, Hamilton Mendes de Figueiredo, Luiza Helena Meller da silva, Antonio Manoel da Cruz Rodrigues. Microbiota of two species of commercially important fish in the Amazon region (Belem-Para-Gayil) Butterfly Peacock Bass (Cichla ocellaris) and Piramutaba (Brachyplatystoma vailantii), African J. Microbiol. Res. 2015; 9(9):572-580.

Egbal OA, Hawa TA, Kalthom EM. Investigating the quality changes of hot smoked clariaslazeraat refrigerated temperature (5°C±1). J. Agri. Food Sci. 2013;1(2):14-20.

Prakash S, Jeyasanta I, Carol R, Patterson J. Microbial quality of salted and sun dried sea foods of tuticorin dry fish market, Southeast Coast of India. Int. J. Microbiol Res. 2011;2(2):188-195.

Hood MA, Ness GE, Blake NJ. Relationship among fecal coliform, Escherichia coli and Salmonella spp. in shellfish. Appl. Environ. Microbiol. 1983; 45(1):122-6.

Mandal SC, Hasan M, Shamsur Rahman M, Manik MH, Mahmud ZH, Sirajul Islam MD. Coliform bacteria in nile tilapia, Oreochromis niloticus of shrimp-gher, pond and fish market. World J. Fish. Marine Sci. 2009;1(3):160-166.

Adams MR, Moses MO. Food Microbiology. (Third edition). The Royal Society of Chemistry, Cambridge, UK; 2008.

Okoro CC, Aboaba OO, Babajide OJ. Quality assessment of a nigerian marine fish, mullet (Liza falcipinnis) under different storage conditions. New York. Sci. J. 2010; 3(8):21-28.

Al-Harbi AH, Uddin MN. Seasonal variation in the intestinal bacterial flora of hybrid tilapia (Oreochromis niloticus, Oreochromis aureus). Cultured in Earthen Ponds in Saudi Arabia Aquaculture. 2004;229: 37–44.

Al Ghabshi A, Al-Khadhuri H, Al-Aboudi N, Al- Gharabi S, Al-Khatri A, Al-Mazrooei N, Sudheesh PS. Effect of the freshness of starting, material on the final product quality of dried salted shark. Advance J. Food Science and Technology. 2012;4(2): 60-66.