Anticoccidial Activity of Allium sativum and Punica granatum against Experimentally Induced Eimeria tenella Infection in Broiler Chickens

Main Article Content

I. R. M. Al- Shaibani
A. M. A. Al- Khadher
A. Z. H. AlHibah

Abstract

This study was conducted to evaluate effect of Allium sativum and Punica granatum against experimentally induced E. tenella infection in broiler chickens. A total of 80 one day old, broiler chickens were purchased and brooded in a deep litter pen. After acclimatization, the birds were allocated into 8 groups (1st to 8th group) separately housed; each group had two replicates hav­ing 5 birds. On 35th day of birds age (day 0 pre infection), birds were weighted individually; dropping samples were collected and after then all birds were infected orally with 36000 sporulated oocysts of E. tenella, oocyst/bird. On  day 7 post infection, 1st & 2nd groups, 3rd & 4th groups, 5th & 6th groups received aqueous extracts of Punica granatum, Allium sativum, and Punica granatum & Allium sativum in combination at doses of (0.7 g/kg, 1.4 g/kg), (0.5 g/kg, 1 g/kg), (0.5 + 0.7 g/kg) and (1.4+ 1 g/kg) respectively for three successive days; while, 7th group given Amprolium at the dose of 1 gm/liter in drinking water and 8th group acts as negative control group and kept untreated. Anticoccidial effect of plant extracts was evaluated on the basis of clinical signs, body weight gain and oocysts counts in faeces. The clinical signs showed by experimental birds were weakness, reduced appetite, ruffled feathers and bloody diarrhea. However, these clinical signs were reduced or disappeared in treated groups. In general, the body weight gain was significantly (p < .05) increased in treated groups compared to control grou p. The higher body weight gain (BWG) value was recorded in 6th group (2624 ±251); whereas; the lower in 3rd group (2264 ±254) on day 21 post infection. Oocysts counts were significant (P ≤ 0.05) drop in treated groups from day 15 and researched the minimum level on day 21 post infection. The findings of the present study revealed that aqueous extracts of Allium sativum and Punica granatum exhibited anticoccidial activities against E. tenella infection in broiler chickens and this may be useful for controlling of E. tenella in poultry.

Keywords:
Anticoccidial, Allium sativum, broilers, Eimeria tenella, Punica granatum.

Article Details

How to Cite
Shaibani, I. R. M. A.-, Khadher, A. M. A. A.-, & AlHibah, A. Z. H. (2020). Anticoccidial Activity of Allium sativum and Punica granatum against Experimentally Induced Eimeria tenella Infection in Broiler Chickens. Asian Journal of Research in Animal and Veterinary Sciences, 5(4), 20-29. Retrieved from https://journalajravs.com/index.php/AJRAVS/article/view/30098
Section
Original Research Article

References

Gueye EF. Village egg and fowl meat production in Africa. World’s Poultry Scientific Journal. 2005;54:73-86.

Abebe E, Gugsa GA. Review on Poultry Coccidiosis. Abyssinia Journal of Science and Technology. 2018;3(1):1-12.

Chauhan S, Roy S. Poultry diseases: Diagnosis and treatment, 3rd ed. New age International ptd, Publishers, New Delhi, India. 2007;152-156.

Conway DP, McKenzie ME. Poultry coccidiosis diagnosis and testing procedures. 3rd ed. Iowa: Blackwell Publishing; 2007.

https://doi.org/10.1002/9780470344620.

McDougald LR. Coccidiosis Diseases of Poultry, 11th ed., , Iowa: Iowa State Press.USA. 2003;1001-1010.

Obasi OL, Ifut OJ, Offiong EA. An outbreak of caecal coccidiosis in a broiler flock post Newcastle disease vaccination. Journal of Animal and Veterinary Advances. 2006; 5(12):1239-1241.

El-Banna HA, El Latif AA, Soliman M. Anticoccidial Activity of Allium Sativum and Aloe Vera in Broilers. International Journal for Agro Veterinary and Medical Sciences (IJAVMS). 2012;7(2):117-125.

Chapman D. Sustainable Coccidiosis control in poultry production: The role of live vaccine. International Journal of Parasitology. 2002;32:617-620.

Udo EJ, Abba AM. Comparative Study of In Vitro Anti-Coccidial Efficacy of Allium Sativum and Carica Papaya. Journal of Zoological Research. 2018;2(2):10-14.

Morris GM, Gasser RB. Biotechnological advances in the diagnosis of avian coccidiosis and the analysis of genetic variation in Eimeria. Biotechnology Advances. 2006;24:590–603.

McDonald V, Shirley MW. Past and future: vaccination against Eimeria. Parasitology. 2009;136:1477–1489.

Tanweer AJ, Saddique U, Bailey CA, Khan RU. Antiparasitic effect of wild rue (Peganum harmala L.) against experimentally induced coccidiosis in broiler chicks. Parasitology Research. 2014;113:2951–2960.

doi:10.1007/s00436- 014-3957-y.

Chand N, Faheem H, Khan RU, Qureshi MS, Alhidary IA, Abudabos AM. Anticoccidial effect of mananoligosa-charide against experimentally induced coccidiosis in broiler. Environmental Science and Pollution Research. 2016; 23:14414–14421.

DOI:10.1007/s11356-016-6600.

Ali M, Chand N, Khan RU, Naz S, Gul S. Anticoccidial effect of garlic (Allium sativum) and ginger (Zingiber officinale) against experimentally induced coccidiosis in broiler chickens. Journal of Applied Animal Research. 2019;47(1):79-84,

DOI: 10. 1080/09712119.2019.1573731.

Blake DP, Tomley FM. Securing poultry production from the ever-present Eimeria challenge. Trends in Parasitology; 2014.

Available:https://doi.org/10.1016/j.pt.2013.10.003.

Acharya KP, Acharya N. Alternatives to fight against coccidiosis: A review. Nepalese Veterinary Journal. 2017;34: 152-167.

Julie DH. Coccidiosis in poultry. Livestock Poultry Health Programs. 1999;2:3-4.

El-Khtam AO, El Latif AA, El-Hewaity MH. Efficacy of turmeric (Curcuma longa) and garlic (Allium sativum) on Eimeria species in broilers. International Journal of Basic and Applied Sciences. 2014;3(3): 349-356.

Allen PC, Fetterer RH. Recent advances in biology and immune biology of Eimeria species and in diagnosis and control of infection with these coccidian par¬asites of poultry. Clinical Microbiology Reviews. 2002;15:58-65.

McDougald LR, Seibert BP. Residual activity of anticoccidial drugs in chickens after withdrawal of med¬icated feeds. Veterinary Parasitolology. 1998;74:91-93.

Youn HJ, Noh JW. Screening of the anticoccidial effect of herb extract against Eimeria tenella. Veterinary of Parasitology. 2001;96:257-263.

Available:http://dx. doi. Org/ 10. 1016/ S0304 - 4017 (01) 00385-5

Chauhan S, Singh VS, Thakur V. Effect of Calotropis procera (madar) and Amprolium supplementation on parasitological parameters of broilers during mixed Eimeria species infection, Veterinary World. 2017;10(8):864-868.

Fleischauer AT, Poole CH, Arab L. Garlic consumption and cancer prevention: meta-analyses of colorectal and stomach cancers. The American Journal of Clinical Nutrition. 2000;72:1047–1052.

Wilson EA, Demming-Adams B. Antioxidant, anti-inflammatory, and antimicrobial properties of garlic and onion. Nutrition and Food Science. 2007;37:178-183.

Faria A, Conceic¸˜ao C. The bioactivity of pomegranate: impact on health and disease. Critical Reviews in Food Science and Nutrition. 2011;51(7):626–634.

Abbas RZ, Colwell DD, Gilleard J. Botanicals: An alternative approach for the control of avian coccidiosis. World Poultry Science Journal. 2012;68:203-215.

Dar PV, Ashfaque M, Zargar AA, Mir IA. Effect of Garlic Extract on haematobiochemical changes in Eimeria tenella Infected Broiler Chicken. National Academy Science Letters; 2014.

DOI:10. 1007/s40009-014-0237-4

Udo EJ, Abba AM, Abdulhamid Y, Mudassir I. In-vivo evaluation of anti-coccidial efficacy of aqueous extract of Allium sativum and Carica papaya in Experimentally Infected Broiler Chicks. Current Trends in Biomedical Engineering & Biosciences. 2018;16(5). ID. 555946.

Waqas M, Akhtar R, Akbar H, Lateef M, Rashid I, Ijaz M. Evaluation of anticoccidial activity of different extraction products of Allium sativum (garlic) in broilers. Journal of the Hellenic Veterinary Medical Society. 2018;69(3):1055-1058.

Pop LM, Varga E, Coroian M, Nedișan ME, Mircean V, Dumitrache MO, Farczádi L, Fülöp I, Croitoru MD, Fazakas M, Gyӧrke A. Efficacy of a commercial herbal formula in chicken experimental Coccidiosis. Parasites Vectors. 2019;12:343.

Parekh J, Chanda S. Antibacterial and phytochemical studies on twelve species of Indian medicinal plants. African Journal of Biomedical Research. 2006;10:175-181.

Agaie BM, Onyeyili PA. Anthelmintic activity of the crude aqueous leaf extracts of Anogeissus leiocarpus in sheep. African Journal of Biotechnology. 2007; 6(13):1511-1515.

Holdswort PA, Conway DP, McKenzie ME, Dayton AD, Chapman HD, Mathis GF, Skinner JT, Mundt HC, Williams RB. World association for the advancement of veterinary parasitology (WAAVP) guidelines for evaluating the efficacy of anticoccidial drugs in chickens and turkeys. Veterinary. Parasitololgy. 2004; 121:189-212.

Nghonjuyi NW, Kimbi HK, Keambou CT, Manka’a CN, Toukala JP, Juliano RS, Lisita FAssessment of anti-coccidial efficacy of ethanolic extract of Aloe vera leaf in Kabir chicken in Cameroon. The Journal of Advances in Parasitology. 2015; 2(2):23-29.

Kim DK, Lillehoj HS, Lee SH, Jang SI, Lillehoj EP, Bravo D. Dietary Curcuma longa enhances resistance against Eimeria maxima and Eimeria tenella infections in chickens. Poultry Science. 2013;92:2635–43.

Masood S, Abbas RZ, Iqbal Z, Mansoor MK, Sindhu ZUD, Zia M A, et al. Role of natural antioxidants for the control of coccidiosis in poultry. Pakistan Veterinary Journal; 2013;33:401–7.

Mahmood A, Khan MA, Khan MN, Qudoos A. Effect of ionophores n some parameters of broilers experimentally infected with Eimeria species. International Journal of Agriculture and Biology. 2001; 3(4):469-471.

Biu AA, Yusuf SD, Rabo JS. Use of neem (Azadirachta indica) aqueous extract as a treatment for poultry coccidiosis in Borno State, Nigeria. African Scientist. 2006; 7(3):147-153.

Tan GH, Long K. Preliminary study of anticoccidial activity of medium chain fatty acids (MCFA) and their corresponding monoglycerides on broiler chicken coccidiosis. Int’l J. Biotechnol. Wellness Indust. 2012;1:134-141.

Zaman MA. Iqbal Z, Abbas RZ, Khan MN. Anti-coccidial activity of herbal complex in broiler chick¬ens challenged with Eimeria tenella. Parasitology. 2011;139(2):237-243.

Muthamilselvan T, Kuo T, Wu Y, Yang W. Herbal remedies for coccidiosis control: A review of plants, compounds, and anticoccidial actions. Evidence-based Complementary and Alternative Medicine. 2016;1-19.

DOI: 10.1155/2016/2657981

Khan RU, Nikousefat Z, Tufarelli V, Naz S, Javdani M, Laudadio V. Garlic (Allium sativa) supplementation in poultry diet: effect on production and physiology. World’s Poultry Science Journal. 2012; 68:417– 424.

DOI:10.1017/ S0043933912000530.

Adibmoradi M, Navidshad B, Seifdavati J, Royan M. Effect of dietary garlic meal on histological structure of small intestine in broiler chickens. The Journal of Poultry Science. 2006;43:378–383.

DOI:10.2141/jpsa.43.378.

Catao RMR, Antunes RMO, Arruda TA, Pereira MSV, Higino JS, Alves JÁ. In vitro antimicrobial activity of the ethanol extract of Punica granatum Linn. (Pomegranate) on outpatient isolates of Staphylococcus aureus. Brazilian Journal Clin. Anal. 2006; 38:111-114.

Włosek AA, S´wia˛tkiewicz S. The effect of a dietary herbal extract blend on the performance of broilers challenged with Eimeria oocysts. Journal of Animal and Feed Sciences. 2012;2:133–142.

Sikkema J, Bont JAM, Poolman B. Mechanisms of membrane toxicity of hydrocarbons. Microbiological Reviews. 1995;59:201–222.

Allen PC, Danforth HD, Augustine PC. Diet modulation of avian coccidiosis. International Journal for Parasitology. 1998;28:1131–1140.