Main Article Content
This study was conducted to determine the effect of Lawsonia inermis leaf meal on growth performance and blood profile of broiler chicks for fifty-six days. A total of 150 day old broiler chicks were used. They were divided into five treatment groups of three replicates each (T1, T2, T3, T4, and T5 fed 0, 10, 20, 30 and 40 g/kg Lawsonia inermis leaf meal respectively). Each replicates contained 10 birds. The experimental design used for this study was complete randomized design. The responses of the broiler to the dietary treatments were measured by feed intake (g/bird), final weight (g/bird), feed conversion ratio (FCR), haematology parameters and serum biochemistry parameters. Result obtained from this experiment indicated that Lawsonia inermis leaf meal had significant (P<0.05) effect on blood profile and growth performance of the broiler chicken at both starter and finisher phase. At the starter phase, the Average weight, Daily weight gain and Final weight had their highest values from broiler birds on T4 fed (30 g/kg Lawsonia inermis leaf meal) while their highest values were observed from broiler birds on T5 fed (40 g/kg Lawsonia inermis leaf meal) at finisher phase. Meanwhile, the feed conversion ratio had its lowest value in T4 at starter phase and T5 at finisher phase. At both starter and finisher phase, Lawsonia inermis leaf meal had Significant (P<0.05) effect on white blood cells (WBC), red blood cells (RBC), haemoglobin (Hb) and Pack cell volume (PCV) which could indicate a boost in the body’s ability to fight of disease. Also, all the serum parameters were significantly affected by dietary treatment of Lawsonia inermis leaf meal at the starter phase while only total protein, albumin, globumin and AST were significantly affected at finisher phase. However, total cholesterol was observed to be reduced among broiler chicks on T4 and T5 (fed 30 g/kg and 40 g/kg of Lawsonia inermis leaf meal respectively) at starter phase. The study therefore concluded that, Lawsonia inermis leaf meal could be included to the diet of broiler chicks up to 40 g/kg without any deleterious effect.
Oluremi OIA, Wuanor GJ, Orayaga KT. Effect of fermentation on some chemical constituents in sweet orange (Citrus sinensis) peal meal and nutrient digestibility in broiler chickens. Proceeding of 14th Annual Conference of Association of Animal Science of Nigeria.-Lautech. 2009;118-120.
Nkwocha GA, Anukam KU, Nkwocha UI. Growth response and carcass characteristics of broiler finisher chickens fed varying dietary levels of sun-dried animal plasma. Proceeding of 14th of Animal Science Association of Nigeria (ASAN)-Lautech. 2009;375-378.
Muthumani P, Meera R, Sundaraganapathy DP, Mohamed ASA, Cholarja K. Biological evaluation of dried fruits of Lawsonia inermis. J Pharm Biomed Sci. 2010;1:1-5.
Donkor SC, Quainoo AK, Gustav M. Propagation of Henna (Lawsonia inermis) cuttings using nathelene acetic acid, indole-3- butyric acid and wood ash. JPBAS. 2013;1:115-23.
Singh A, Singh DK 2001. Molluscicidal activity of Lawsonia inermis and it binary and tertiary combinations with plant derived molluscicides. Indian Journal Experimental Biology. 2001;263-268.
Azaizeh H, Fuider S, Said K, Khalil O. Ethno-botanical knowledge of local Arab practitioners JV, Lewis SM in the Middle Eastern region. Journal of Filoterapia. 2003;74:98-108.
Varghese JK, Silvipriya K, Resmi S, Jolly C. Lawsonia inermis (Henna): A natural dye of various therapeutic uses - A review. Inventi Rapid: Cosmeceuticals. 2010;1:1-5.
Abdulmoneim MA. Evaluation of Lawsonia inermis Linn. (Sudanese Henna) leaf extract as an antimicrobial agent. Research Journal of Biological Sciences. 2007;2:417-423.
Dacie. Practical haematology 7th (ED). ELBS with Churchill Livingstone. England. 1991;37-85.
Jain NC. Schalm’s veterinary hematology. 8th edition lea and febiger, Philadephia; 2000.
Cholada VC. Mean corpuscular volume (MCV); 2012.
Retrieved: December 14, 2017
Gernsten T. Medline plus Medical Encyclopedia; RBC indices; 2009.
Available at;en.wikipedia.org/wiki/red blood cell indices
Retrieved: December 12, 2017
Doumas BT. Standards for total serum protein assay. Clinical Chemistry. 1975; 21:1159.
Doumas BT, Biggs HG. Determination of serum albumin. In G.R. Cooper (Ed.), Standard Methods of Clinical Chemistry. New York: Academic Press. 1972;175.
Allain CC, Poon LS, Chon CGS, Richmond W, Fu PC. Enzymatic determination of total serum cholesterol. Clinical Chemistry. 1974;20:470.
Kaplan A, Szabo LL. Clinical Chemistry: Interpretation and Techniques, 2nd (Ed). Philadelphia: Lea and Ferbiger. 1983;313-314.
Okorie SU, Ekwe CC. The comparative analysis of sprouted legume and Cereal based composite diet. Journal of Applied Biotechnology & Bioengineering. 2017;4 (2):554‒561.
Sharifi SD, Khorsandi SH, Khadem AA, Salehi A, Moslehi H. The effect of four medicinal plants on the performance, blood biochemical traits and ileal microflora of broiler chicks. Journal of Veterinary. 2013;83(1):69-80.
Nihad AA, Fadel RA, AL-Kafagy AK (2006). Effect of adding different levels of the nutrient powder to the ration in some physiological characteristics of the blood of broiler chickens. International Journal of Current Research and Academic Review. 2006;4:34-39.ISSN: 2347-3215
Shinde NS, Barmase BS, Ambulkar DR, Rekhate DH. Effect of probiotic and enzyme supplement on the performance of broilers. Indian Poultry Science Association XXIII Conference & National Symposium. Hydrabad. 2005;2:83-84.
Kabir SML, Rahman MM, Rahman MB, Ahmed SV. The dynamics of probiotics on growth performance and immune response in broilers. International Journal of Poultry Science. 2004;3(5):361-364.
Dhekane VS. Effect of supplementation of probiotic on performance of broilers. M.V.Sc. Thesis Submitted to MAFSU, Nagpur; 2005.
Ogunwole OA, Kolade EO, Taiwo BA. Performance and carcass characteristics of broilers fed five different commercial vitamin-mineral premixes in Ibadan, Nigeria. International Journal of Poultry Science. 2012;11(2):120-124.
Denil M, Okan F, Celik K. Effect of dietary probiotic, organic acid and antibiotic supplementation to diets on broiler performance and carcass yield. Pakistan Journal of Nutrition. 2003;2:89-91.
Tollba AAH, Hassan MSH. Using some natural additive to improve physiological and productive performance of broiler chicks under high temperature condition. Black cumin (Nigella sativa) or garlic (Allium sativum). Poultry Science. 2003; 23:327-40.
Nikola P, Dragomir L, Vidica S, Ljiljana K, Milos B, Dragana L, Sladana Z. Effect of spice herbs in broiler chicken nutrition on productive performance. Journal of Veterinary Medicine. 2014;78(2):171- 177.
Mitruka BJ, Rawnley HM. Clinical biochemical and hematological reference values in normal experimental animal 2nd edition Masson Publishing USA. Inc.1961; 1977.
Anon. Guide to the care and use of experimental animals (Canadian council on animal care, Ottawa, Ontario, Canada. 1980;1:85–90.
Schalm OW, Jain NC, Carol EJ. Veterinary Haematology. 3rd Edition. Publisher; Lea and Febiger Philadelphia; 1975.
Soetan KO, Akinrinde AS, Ajibade TO. Preliminary studies on the hematological parameters of cockerels fed raw and processed guinea corn (Sorghum bicolor), Proceedings of 38th Annual Conference of Nigerian Society for Animal Production. 2013;49-52.
Tijani LA, Akanji AM, Agbalaya K, Onigemo M. (Haematological and serum biochemical profiles of broiler chickens fed diets containing moringa leaf meals. Journal of Tropical Agriculture, Food, Enviroment and Extension. 2015;14(3):7-11
Kwiterovich PO. The metabolic pathways of high-density lipoprotein, low-density Lipoprotein, and triglycerides: a current review. The American journal of cardiology. 2000;86(12A):5L–10L. PMID: 11374859.
Thrall MA, Veiser G, Allison R, Campbel TW. Veterinary Hematology and Clinical Chemistry. 2nd Edition. 2012;60-90.