Risk Factors and Antimicrobial Resistance of Bacillus cereus Isolated from Bovine Raw Milk and Fecal Matter from Dairy Farms in Khartoum Bahri Locality, Sudan
Majda I. A. Mohammadin
Department of Food Safety and Veterinary Public Health, College of Veterinary Medicine, University of Bahri, Sudan.
Adil M. A. Salman
Chairman of One Health Center, University of Bahri, Sudan.
El Ayis A. Abubaker *
Department of Internal Medicine, Pharmacology and Toxicology, College of Veterinary Medicine, University of Bahri, Sudan.
Hamad Iman M.
Department of Physiology, College of Medicine, University of Bahri, Sudan.
*Author to whom correspondence should be addressed.
Abstract
This study was conducted in Khartoum Bahri locality during the period from 2020 to 2021. A total of 40 random samples of raw milk and fecal matter were collected from different dairy farms in Bahri locality with the objectives to isolate Bacillus Cereus organism and to illustrate the resistance pattern of antimicrobial to confirm B. cereus. A questionnaire was designed to show the cleanness, mastitis and fecal matter removal status of the farms. Samples were examined for the prevalence of B. cereus. Results of the bacteriological methods were confirmed using PCR. The data showed that B. cereus was isolated from 9(45%), 15(75%) of the raw milk, and fecal matter, respectively. In farm (1) the prevalence rate of B. cereus in raw milk was 4(20%) which was the highest among other farms, 2(10%), 2(10%), 1(5%) in farm2, farm3 and farm 4, respectively. The isolated organisms were found to be highly resistant to Ampicillin\ Sulbactam, Cotrimoxazole, Cefotaxime, Cloxacillin, Colistin and Ceftriaxone and sensitive to Cephalexin, Tetracyclin, Ciprofloxacin, Levofloxacin, streptomycin, Roxithromycin, and Gentamicin.
Keywords: Bacillus cereus, antimicrobial susceptibility, PCR