Sequencing and Phylogenetic Study of Bovine Papilloma Virus
J. D. Chavan *
Department of Animal Husbandry, KNP College of Veterinary Sciences, Shirval, Maharashtra, India.
D. M. Muglikar
Department of Veterinary Microbiology, KNP College of Veterinary Sciences, Shirval, Maharashtra, India.
Abhijit Barate
Department of Veterinary Biochemistry, KNP College of Veterinary Sciences, Shirval, Maharashtra, India.
U. M. Tumlam
Department of Veterinary Microbiology, KNP College of Veterinary Sciences, Shirval, Maharashtra, India.
V. S. Dhaigude
Department of Veterinary Pathology, KNP College of Veterinary Sciences, Shirval, Maharashtra, India.
M. S. Budhe
Department of Veterinary Microbiology, KNP College of Veterinary Sciences, Shirval, Maharashtra, India.
P. P. Mhase
Department of Veterinary Microbiology, KNP College of Veterinary Sciences, Shirval, Maharashtra, India.
*Author to whom correspondence should be addressed.
Abstract
The involvement of different genotype of BPV was reported from different parts of world. In this study a total of 5 BPV-1-positive PCR products were sequenced to determine the type of viral genome. The Delta papillomavirus sp. L1 generating genes showed the most similarity in a blast search (99.66 percent). Furthermore, multiple sequence alignment revealed 99% and 99.7 % identity with Indian sequences KF055288.1 (IVRI, India) and HG918265.1 (IVRI, India) respectively. Phylogenetic analysis of partial sequences from the current study revealed that they are closely connected to MN977321.1 (Italy) and AB626705.1 (Japan), but are distantly related to KX907623.1 (China) and KY372394.1 (Turkey) sequences.
Keywords: BPV, Sequencing, cattle, histopathology, phylogeny