Life Cycle of Hyalomma dromedarii Ticks (Acari: Ixodidae) on Sheep under Experimental Conditions

Khalid M. Taha

Animal Resources Research Corporation, P.O.Box-610, Al Amarat, Khartoum, Sudan.

Wisal A. Mustafa

Directorate of Animal Resources, Eldamer, River Nile State, Sudan.

Sara B. Mohammed *

College of Veterinary Medicine, Sudan University of Science and Technology, Sudan.

Mohamed O. Hussien

Department of Microbiology, Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, Sudan.

*Author to whom correspondence should be addressed.


Aims: This experiment aimed to study the life cycle of Hyalomma dromedarii fed on sheep under experimental conditions.

Place and Duration of Study: The study was conducted between June and October 2018 at Atbara Veterinary Research Laboratory, Atbara, River Nile state, Sudan.

Methodology: Nine sheep were purchased from the local market and used as hosts for feeding H. dromedarii (larvae, nymphs and adults) using the ear bags method. After feeding, detached engorged larvae, nymphs and adults were maintained under laboratory conditions at 25°C and relative humidity (R.H) 75%. All information including feeding periods, the number of engorged and detached ticks (larvae, nymphs and adults), pre-moulting and moulting periods, preoviposition and preeclosion periods were recorded daily.

Results: 93.3% of larvae (1400/1500) successfully complete their feeding during a period between 3-17 days at 34.5°C and (R.H) 47%. The pre-moulting and moulting periods of larvae were between 12 -13 days and 2-3 days, respectively.

Regarding the nymph stage, 96% of nymphs (288/300) completed their feeding between 3-10 days at 27.4°C and (R.H) 56%. The nymph took between 13-15 days as pre-moulting period, while the moulting period was 3-4 days. All adult ticks, 100% (30/30) completed their feeding successfully on sheep during a period between 4-10 days at temperatures ranging between 33-35°C and (R.H) ranging between 36-41%. The preoviposition and preeclosion period varied between 4-6 days and 16-21 days, respectively. The average of engorged ticks' weight, number of eggs produced and the weight of mass eggs were 396.6 mg, 3530 eggs and 217.84 mg, respectively. Regarding hatchability, the results revealed that more than 97.3% of eggs were able to hatch and produce larvae.

Conclusion: This study showed that H. dromedarii behaved as a three-host tick and completed its life cycle between 94- 140 days. The results confirmed the positive relationship between the weight of the engorged female and the number of egg laid, as increases in the females’ weight were associated with an increase in the number of eggs.

Keywords: Hyalomma dromedarii, life cycle, sheep, Sudan

How to Cite

Taha, K. M., Mustafa, W. A., Mohammed, S. B., & Hussien, M. O. (2022). Life Cycle of Hyalomma dromedarii Ticks (Acari: Ixodidae) on Sheep under Experimental Conditions. Asian Journal of Research in Animal and Veterinary Sciences, 5(3), 192–200. Retrieved from


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MARFR. Ministry of Animal Resources, Fisheries and Rangelands. Annual Report, Sudan Government. Sudan; 2016.

Wilson RT. Livestock in the Republic of the Sudan: Policies, production, problems and possibilities. Anim Husb Dairy Vet Sci. 2018;2.


Jongejan F, Uilenberg G. The global importance of ticks. Parasitology. 2004; 129:S3-S14.

Perveen N, Bin Muzaffar S, Al-Deeb MA. Population dynamics of Hyalomma dromedarii on camels in the United Arab Emirates. Insects. 2020;11(5):320.


Hassan SM. Salih DA. An overview of factors responsible for geographic distribution pattern of ixodid ticks in the Sudan. Sokoto J Vet Sci . 2013;11(2):1-9.

ElGhali A, Hassan SM. Ticks (Acari: Ixodidae) infesting camels (Camelus dromedarius) in Northern Sudan. Onderstepoort J Vet Res. 2009;76: 177-185.

Abdullah HHAM, El-Shanawany EE, Abdel-Shafy S, Abou-Zeina HAA, Abdel-Rahman EH. Molecular and immunological characterization of Hyalomma dromedarii and Hyalomma excavatum (Acari: Ixodidae) vectors of Q fever in camels. Vet World. 2018;11(8):1109-1119.

DOI: 10.14202/vetworld.2018.1109-1119

Ibrahim SAH. Tick and tick-borne diseases of cattle and sheep in North Darfour State, Sudan. Thesis, University of Khartoum, Sudan; 2009.

Salim BOM. Molecular, serological and parasitological detection of piroplasmosis of horses in the Sudan. Thesis, University of Khartoum, Sudan; 2008.

Salih DA, Hassan SM, El Hussein AM, Jongejan F. Preliminary survey of ticks (Acari: Ixodidae) on cattle in Northern Sudan. Onderstepoort J Vet Res. 2004;71:319-326.

Ahmed BM, ElHussein AM, ElKhidir AO. Some observations on ticks (Acari: Ixodidae) infesting sheep in River Nile State of Northern Sudan. Onderstepoort J Vet Res. 2005;72:239-243.

Tomassone L, Grego E, Callà G, Rodighiero P, Pressi G, Gebre S, et al. Ticks and tick-borne pathogens in livestock from nomadic herds in the Somali Region, Ethiopia. Exp Appl Acarol. 2012;56: 391- 401.

Bendary HA, Rasslan F, Wainwright M, Alfarraj S, Zaki AM, Abdulall AK. Crimean-Congo hemorrhagic fever virus in ticks collected from imported camels in Egypt. Saudi J Biol Sci. 2022;29(4):2597-2603.

DOI: 10.1016/j.sjbs.2021.12.043

Hamed MI, Zaitoun AMA, El-Allawy TAA, Mourad IM. Investigation of Theileria camelensis in camels infested by Hyalomma dromedarii ticks in Upper Egypt. J Adv Vet Res. 2011;1:4-7.

D'oliveira C, Van der Weide M, Jacquiet P, Jongejan, F. Detection of Theileria annulata by the PCR in ticks (Acari: Ixodidae) collected from cattle in Mauritania. Exp Appl Acarol. 1997;21: 279-291.

DOI: org/10.1023/A:1018455223462

Hagras AE, Khalil GAM. Effect of temperature on Hyalomma dromedarii Koch (Acari:Ixodidae). J Med Entomol. 1988;25(5):354-359.

Alahmed AM, Kheir S.M. Life cycle and survival of Hyalomma dromedarii (Acari: Ixodidae) under laboratory conditions. Agric Mar Sci. 2003;8:11-14.

Ouhelli H. Comparative development of Hyalomma marginatum (Koch,1844), H. detritum (Schulze, 1919), H. Anatolicum excavatum (Koch, 1844), H. lusitanicum (Koch, 1844) and H. dromedarii (Koch, 1844) under laboratory conditions. Acta Parasitol. 1994;39:153-157.

ELGhali, A. Studies on camel ticks in River Nile State, Sudan with particular reference to ecology and behavior of Hyalomma Dromedarii. Acari: Ixodide. Thesis, Faculty of Veterinary Medicine, University of Khartoum, Sudan; 2005.

ElGhali A, Hassan SM. Drop-off rhythms and survival periods of Hyalomma dromedarii (Acari: Ixodidae) fed on camels (Camelus dromedarius) in the Sudan. Vet Parasitol. 2010;170(3-4): 302-306.


Hoogstraal H. African Ixodoidea. Vol. 1. Ticks of the Sudan (with special reference to equatoria province and with preliminary reviews of the genera Rhipicephalus, Margaropus, and Hyalomma). US Navy, Washington, DC; 1956.

Ahmed BM. Studies on epizoology of theileria ( Lestoquardi nomen novum) hirci (Apicomplexa: Theileriidae) in River Nile State, Sudan. Thesis, Nile Valley University, Sudan; 1999.

Ouhelli H, Pandey VS. Development of Hyalomma lussitanicum under laboratory conditions. Vet Parasit. 1984;15:57-66.

Salimi-Bejestani MR, Changizi E, Darvishi MM. Abnormal life cycle of Hyalomma dromedarii (Acari: Ixodidae) on single-humped camels in semnan, north-east of Iran. Arch Razi Inst. 2016;71:195-198.


Elati K, Bouaicha F, Dhibi M, Ben Smida B, Mhadhbi M, Obara I, et al. Phenology and phylogeny of Hyalomma spp. ticks infesting one-humped camels (Camelus dromedarius) in the Tunisian Saharan Bioclimatic Zone. Parasite. 2021;28:44.

Apanaskevich DA. Host-parasite relationships of the genus hyalomma koch, 1844 (Acari, Ixodidae) and their connection with microevolutionary process. Parazitologiia. 2004;38:515-523.

Siroký P, Erhart J, Petrželková KJ, Kamler M. Life cycle of tortoise tick Hyalomma aegyptium under laboratory conditions. Exp Appl Acarol. 2011;54(3):277-284.


Jones CR, Brunner JL, Scoles GA, Owen JP. Factors affecting larval tick feeding success: Host, density and time. Parasit Vectors. 2015;8:340.


Gray JS, Dautel H, Estrada-Peña A, Kahl O, Lindgren E. Effects of climate change on ticks and tick-borne diseases in europe. Interdiscip Perspect Infect Dis, 2009:593232.

DOI: 10.1155/2009/593232

Afshari A, Habibi G, Abdigoudarzi M, Yazdani F. Establishment and validation of theileria annulata sporozoite Ak-93 infection in laboratory-reared Hyalomma anatolicum tick using in vivo and In vitro assays. J Arthropod Borne Dis. 2020;14(3):261-269. v14i3.4559

Elhachimi L, Valcárcel F, Olmeda AS, Elasatey S, Khattat SE, Daminet S, et al. Rearing of Hyalomma marginatum (Acarina: Ixodidae) under laboratory conditions in Morocco. Exp Appl Acarol. 2021;84:785-794.


Ahmed BM, Taha KM, El Hussein AM. Life cycle of Hyalomma anatolicum Koch, 1844 (Acari: Ixodidae) fed on rabbits, sheep and goats. Vet Parasitol. 2011;177(3-4): 353-358.


Gargili A, Thangamani S, Bente D. Influence of laboratory animal hosts on the life cycle of Hyalomma marginatum and implications for an In vivo transmission model for Crimean-Congo hemorrhagic fever virus. Front cell infect microbial. 2013;3:39.


Hadi UK, Adventini, M. Fecundity, Oviposition and egg incubation period of female Rhipicephalus Sanguineus Latreille (Acari: Ixodidae) ticks in Indonesia. J Vet Med Res. 2015;2 (5):1036.